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macsmixtm tube rotator alternative miltenyi biotec  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec macsmixtm tube rotator alternative miltenyi biotec
    Macsmixtm Tube Rotator Alternative Miltenyi Biotec, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 920 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rotating/gentleMACS+Octo+Dissociator+with+Heaters/pm42406597-96-221-225
    Average 97 stars, based on 920 article reviews
    macsmixtm tube rotator alternative miltenyi biotec - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Incubation:

    Article Title: Downregulation of STAT3 transcription factor reverses synaptotoxic phenotype of reactive astrocytes associated with prion diseases
    Article Snippet: Brain tissues were enzymatically dissociated using the Adult Brain Dissociation Kit (#130-107-677, Miltenyi Biotec) according to the manufacturer’s instructions. .. Briefly, the cortical brain tissues were dissected, transferred into gentleMACS C tubes (#130-093-237, Miltenyi Biotec) containing an enzyme mixture and incubated on a rotating gentleMACS Octo Dissociator (Miltenyi Biotec) for 30 min. ..

    Article Title: Downregulation of STAT3 transcription factor reverses synaptotoxic phenotype of reactive astrocytes associated with prion diseases.
    Article Snippet: Brain tissues were enzymatically dissociated using the Adult Brain Dissociation Kit (#130-107-677, Miltenyi Biotec) according to the manufacturer’s instructions. .. Briefly, the cortical brain tissues were dissected, transferred into gentleMACS C tubes (#130-093-237, Miltenyi Biotec) containing an enzyme mixture and incubated on a rotating gentleMACS Octo Dissociator (Miltenyi Biotec) for 30 min. ..

    Isolation:

    Article Title: Mucosal-associated invariant T cells contribute to suppression of inflammatory myeloid cells in immune-mediated kidney disease.
    Article Snippet: IgG1k isotype (200 μg per injection, Biolegend, clone MOPC-21) was used as control and injected in the same manner. .. For isolation of renal leukocytes, mouse kidneys were cut into small pieces and were enzymatically digested in complete medium (RPMI 1640, 10% Fetal Bovine Serum, 1%HEPES, 1%Penicillin/Streptomycin; all Gibco, Thermo Fisher Scientific, USA) supplemented with Collagenase D (0.4mg/ml, Roche, Basel, Switzerland) and DNase I (100 μg/ml, Roche) for 45min at 37 °C while rotating on a MACSmix® tube rotator (Miltenyi, Bergisch Gladbach, Germany). .. After further dispersion with the gentleMACS® dissociator (Miltenyi), leukocytes were purified by Percoll gradient centrifugation (37.5%) (GE Healthcare, Chicago, USA) and further enriched by subsequent erythrocyte lysis with ammonium chloride.

    Article Title: Mucosal-associated invariant T cells contribute to suppression of inflammatory myeloid cells in immune-mediated kidney disease
    Article Snippet: IgG1k isotype (200 μg per injection, Biolegend, clone MOPC-21) was used as control and injected in the same manner. .. For isolation of renal leukocytes, mouse kidneys were cut into small pieces and were enzymatically digested in complete medium (RPMI 1640, 10% Fetal Bovine Serum, 1% HEPES, 1% Penicillin/Streptomycin; all Gibco, Thermo Fisher Scientific, USA) supplemented with Collagenase D (0.4 mg/ml, Roche, Basel, Switzerland) and DNase I (100 μg/ml, Roche) for 45 min at 37 °C while rotating on a MACSmix® tube rotator (Miltenyi, Bergisch Gladbach, Germany). .. After further dispersion with the gentleMACS® dissociator (Miltenyi), leukocytes were purified by Percoll gradient centrifugation (37.5%) (GE Healthcare, Chicago, USA) and further enriched by subsequent erythrocyte lysis with ammonium chloride.

    other:

    Article Title: LncRNA‐MEG3 Regulates Muscle Mass and Metabolic Homeostasis by Facilitating SUZ12 Liquid–Liquid Phase Separation
    Article Snippet: Mitochondrial oxygen consumption in GAS muscle fibers isolated from lncRNA‐MEG3 knockdown and overexpression mice was measured using an Oxygraph‐2k units (Oroboros Instruments, Innsbruck) at 25 °C.



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